A canvas collects a number of video images and two-photon stacks into a motor space. A canvas represents a single imaging session. An imaging session can be (i) all images taken during a single in vivo imaging session of an animal, (ii) all images taken while imaging one in vitro brain-slice.

Note: A canvas can (in theory) be created from any collection of stacks if you are using a motorized (and computer controlled) objective. For now, canvas creation is possible when using ScanImage and an mp285 motor controller from Sutter Instruments.

Keyboard Shortcuts

Keystroke Action
Arrow Keys Pan
Shift + Arrow Keys Pan video layer only
+/- Zoom in/out
Shift + / Shift – Zoom in/out (smaller steps)
C Left and top side panels to display list of video/stacks, set contrast
M Show motor interface. Only available when Canvas is viewed with mp285

Additional Features

canvas_tie2A canvas from an in vivo imaging session. stack_browser_tie2The Canvas and Stack Browser are tightly integrated allowing you to view all your 3D volumes from multiple imaging sessions.
canvas_ cerebellum_1A canvas from an imaging session with a cerebellar slice. The gray images are taken with a video camera. The position of the acquired two-photon stacks are represented with green squares. canvas_ cerebellum_2The same canvas zoomed in on the area that was images with two-photon.
canvas_ cerebellum_toolsThe same canvas with the canvas tools displayed. The canvas tools provides a list of all objects in a canvas. Selecting an object will highlight the object in the Canvas and the Stack Browser.

A canvas has multiple layers, one layer for video and another for two-photon stacks. The layers can be nudged left/right and up/down so that the images taken on different light paths can be aligned.

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